FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_92.3510000005c9fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_92.3510000005c9fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84495
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT1050.021.01125721
TTTTCTG601.022272E-921.01125521
ATTTCTG405.288557E-621.01125520
CAGCCAC1400.020.9988172
CTCTGGA5700.020.9988178
TTGTCTG354.5401044E-520.99881718
CTCTGCA2900.020.9988178
CTCTGAT3750.020.9988178
CTCTGAG5450.020.9988178
CTCTGAA3250.020.9988178
CTGAGGA405.315813E-620.99881710
CTGAGCC405.315813E-620.99881710
CCAGTTT354.5401044E-520.99881717
TTTCTTT354.5401044E-520.99881717
TTCGCCA2800.020.9988171
AGGATTT354.5401044E-520.99881717
GTACTTT354.5401044E-520.99881717
TCTGGTA1400.020.9988179
TATGTTT405.315813E-620.99881717
CTGGGAA354.5401044E-520.99881710
Produced by FastQC (version 0.11.2)