FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_91.3510000005c9d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_91.3510000005c9d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51856
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA4600.8870718913915459No Hit
CTAGCTGGATGTCTGTCTCTTATACAC1350.2603363159518667No Hit
CTAGCTGGATCTGTCTCTTATACACAT820.15813020672631903No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA580.11184819500154274No Hit
CTAGCTGGATGTGGGGCTGTCTCTTAT550.1060629435359457No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA550.1060629435359457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGAT1300.021.0000024
GCTGGAT50200.021.0000024
CCGTTTT651.1823431E-1021.00000218
ATGGATG1300.021.0000025
ATGTGAA1300.021.0000029
TGCTTTT651.1823431E-1021.00000218
AGATGGA1300.021.0000023
TGTGGGA651.1823431E-1021.00000210
CTAGATG1300.021.0000021
ATGTAGA651.1823431E-1021.0000029
TGTTGTT303.8642192E-421.010
AGGGGTT354.49477E-521.016
TTGTCTG250.003337026321.018
TGGTTTT900.021.018
GGATGTG22650.021.07
GGATGTC9250.021.07
TCCTTTT250.003337026321.018
TGTGACA250.003337026321.010
TGTTGGC354.49477E-521.010
TGTTGCG354.49477E-521.010
Produced by FastQC (version 0.11.2)