FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_89.3510000005c99f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_89.3510000005c99f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73835
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC3020.40902011241281233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC405.2715386E-621.01220321
TTTTTTC303.8704913E-421.01220321
GTTTCTG701.4551915E-1121.01220320
TCAACCG67100.021.0122031
TCTACCG1350.021.0122031
TCTACAC250.003339019621.01220321
TCAAACG900.021.0122031
TCCACCG1500.021.0122031
TTCTACA250.003339019621.01220320
TCACCCG1000.021.0122031
TTAACCG1500.021.0122031
ATTTCTG651.200533E-1021.01220320
CTTCTGT250.003339019621.01220320
AGGGGTG701.4551915E-1120.99796910
AGCTTTT354.5315297E-520.99796918
TAGGATA1250.020.9979699
TAGGAGG1250.020.9979699
TAGGAGC1400.020.9979699
TAGGACC1100.020.9979699
TAGGACA850.020.9979699
Produced by FastQC (version 0.11.2)