FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_86.3510000005c932.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_86.3510000005c932.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27047
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA940.34754316560062115No Hit
TGTTGCCGAACAGGGGGGGGTTTTTTT290.10722076385551078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG250.003296171321.00000212
ACAGTGA250.003296171321.00000210
TGATGCC558.04539E-921.0000021
ACAGTCG250.003296171321.00000210
ACAGCAG250.003296171321.00000210
AGGGGGT250.003296171321.00000212
AACAATA250.003296171321.0000029
ACACGGT250.003296171321.00000210
ACACGAG250.003296171321.00000210
GCCTTTT558.04539E-921.00000218
AACAAGT250.003296171321.0000029
ACGTTTT250.003296171321.00000218
TGATTTT506.899609E-821.00000218
TTCTGTC1000.021.00000221
TGCTGTC250.003296171321.00000219
GATGCCG558.04539E-921.0000022
GAGTTTT558.04539E-921.00000218
ACATTGG250.003296171321.00000210
CGGGTTT250.003296171321.00000217
GGCCTTT250.003296171321.00000217
Produced by FastQC (version 0.11.2)