FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_85.3510000005c918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_85.3510000005c918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43436
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1050.2417349663873285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT850.021.03695318
CGTTTTT3400.021.03695319
TGGTTTT1200.021.03695118
TCCTTTT455.996262E-721.03695118
GTGTTTT1350.021.03695118
GCCTTTT701.2732926E-1121.03695118
AGCTTTT651.1277734E-1021.03695118
ACGTTTT609.658834E-1021.03695118
TGTCTGT250.003294476521.03695119
TTATTTT1200.021.03695118
TGATTTT651.1277734E-1021.03695118
CGCTTTT609.658834E-1021.03695118
TGTTTTT4150.021.03695119
CAATTTT701.2732926E-1121.03695118
GTTTCTG354.412518E-521.03695120
CATTTTT2000.021.03695119
CCGTTTT800.021.03695118
TAGTTTT701.2732926E-1121.03695118
TGCTTTT651.1277734E-1021.03695118
CCATTTT405.136182E-621.03695118
Produced by FastQC (version 0.11.2)