FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_84.3510000005c8fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_84.3510000005c8fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80682
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA2740.339604868496071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTT303.8900666E-421.00000216
TCCTTTT303.8900666E-421.00000218
GTCATTT303.8900666E-421.00000217
CAGTCCG1200.021.0000029
CAGTTAA303.8900666E-421.0000029
TGACTTT303.8900666E-421.00000217
CCCGGTT303.8900666E-421.00000216
AGTAGAT303.8900666E-421.00000210
TGATCTG303.8900666E-421.00000218
CAATTTT601.02591E-921.00000218
AGTGGAG1200.021.00000210
CCGTTTT950.021.00000218
CGGATTT303.8900666E-421.00000217
AGAATTT303.8900666E-421.00000217
AGTATAG303.8900666E-421.00000210
GTGGGAC303.8900666E-421.00000211
AGTGCCG601.02591E-921.00000210
GCGCTTT601.02591E-921.00000217
AGTGTCG601.02591E-921.00000210
AGTCGTC303.8900666E-421.00000210
Produced by FastQC (version 0.11.2)