FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_83.3510000005c8d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_83.3510000005c8d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81798
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA5030.6149294603780043No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA840.10269199735934864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACAA354.481015E-521.0330891
CACAAAA405.237678E-621.0330891
CACCGAA3250.021.0330891
CAAAGAA651.200533E-1021.0330891
CACAGAA72450.021.0330891
CATAGAA1450.021.0330871
CACAAGG354.5235967E-521.0073453
GTTTCTG701.4551915E-1121.00734520
ACACAAG354.5235967E-521.0073452
AAGAAGG651.2187229E-1021.0073453
ACAAGGA354.5235967E-521.0073454
ATTTCTG751.8189894E-1221.00734520
AAAGAAG651.2187229E-1021.0073452
CTTCTGT354.5235967E-521.00734520
CTTTCTG354.5235967E-521.00734520
TAGAAGG1450.021.0073433
ACAAAAG405.2936593E-621.0073432
ACAGAAG72500.021.0073432
AGAAGGA77500.021.0073434
TTTCTGT1800.021.00734321
Produced by FastQC (version 0.11.2)