FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_8.3510000005bf8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_8.3510000005bf8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66725
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC11061.6575496440614463No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC2490.3731734732109404No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC1280.19183214687148745No Hit
ACAGTGCTCATGGCTGTCTCTTATACA960.1438741101536156No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC870.13038591232671412No Hit
ACAGTGCTCCTGTCTCTTATACACATC740.11090295991007869No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC730.1094042712626452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTC456.20179E-721.00000210
GCCTTTT651.200533E-1021.00000218
CATGAGA651.200533E-1021.0000029
ATCTTTT456.20179E-721.00000218
CATGAAA456.20179E-721.0000029
ACAGAGC456.20179E-721.0000021
GCGATTT456.20179E-721.00000217
AATTTTT2600.021.00000219
ACGTTTT651.200533E-1021.00000218
AGGGTTT456.20179E-721.00000217
CAATTTT651.200533E-1021.00000218
ATGGTGT651.200533E-1021.00000210
ATGGTGG1300.021.00000210
ATGGTGC456.20179E-721.00000210
CCGTTTT900.021.00000218
ACATTTT456.20179E-721.00000218
AACTTTT456.20179E-721.00000218
CAGAGCT456.20179E-721.0000022
AGAGCTC456.20179E-721.0000023
CCATTTT456.20179E-721.00000218
Produced by FastQC (version 0.11.2)