FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_79.3510000005c869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_79.3510000005c869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60275
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA1060.1758606387391124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACAT1300.021.0166131
CAGCCAT1900.021.0166111
CAGAGAT354.482911E-521.0166111
CAGATAT303.8536126E-421.0166111
CATACAT751.8189894E-1221.0166111
CAAGACG354.5117664E-520.99917210
CAATGGC701.4551915E-1120.99917210
CAATGCA354.5117664E-520.99917210
GATATTT354.5117664E-520.99917217
GCAAGAA701.4551915E-1120.9991729
ATGATTT354.5117664E-520.99917217
CAACCGG354.5117664E-520.99917210
CAATCTC354.5117664E-520.99917210
AAGGGCG354.5117664E-520.99917211
GCAACAT1050.020.9991729
GCAACAA354.5117664E-520.9991729
TAACTTT354.5117664E-520.99917217
GCAAACT701.4551915E-1120.9991729
GCAAAAA354.5117664E-520.9991729
CGGTGTT354.5117664E-520.99917216
Produced by FastQC (version 0.11.2)