FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_76.3510000005c80c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_76.3510000005c80c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53628
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC2400.44752741105392707No Hit
AGAGGACGAGCTGTCTCTTATACACAT1870.3486984411128515No Hit
AGAGGACGAGATCTGTCTCTTCTACAC940.17528156932945477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT609.949872E-1021.0149418
GGCATTT250.003324705221.0149417
AGCTTTT558.440111E-921.0149418
AAATTTT405.2211435E-621.0149418
TTCGTTT250.003324705221.0149417
AATTTTT1600.021.0149419
GTCGTTT507.1751856E-821.0149417
AATTTCT250.003324705221.0149419
GTTCTTT250.003324705221.0149417
GGTCTTT303.8479874E-421.0149417
TTATTTT405.2211435E-621.0149418
CTCTTTT558.440111E-921.0149418
GCGGTTT405.2211435E-621.0149417
GTTTTCT250.003324705221.0149420
CCCATTT250.003324705221.0149417
ACATTTT303.8479874E-421.0149418
ACTTTTT1450.021.0149419
TACACAT250.003324705221.0149421
AACTTTT405.2211435E-621.0149418
CTGGTTT405.2211435E-621.0149417
Produced by FastQC (version 0.11.2)