FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_75.3510000005c7e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_75.3510000005c7e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57056
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAA52250.021.0000023
TTAGACC601.0077201E-921.01
AGGGGTT250.003341092321.016
CGACACG303.8708036E-421.010
GAGGCGG354.5049794E-521.011
GAGGCGC250.003341092321.011
GAGGTGG303.8708036E-421.011
GAGGCCG250.003341092321.011
TGCATTT250.003341092321.017
AGGGGGG250.003341092321.012
AGGGGCG303.8708036E-421.012
GTGTCTT250.003341092321.016
GTGTTTT1850.021.018
GGGTTTT2400.021.018
AATCTTT250.003341092321.017
CCAACGA56750.021.06
GGCATTT250.003341092321.017
CGATATT354.5049794E-521.010
CGATATG303.8708036E-421.010
CGATAGT303.8708036E-421.010
Produced by FastQC (version 0.11.2)