FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_74.3510000005c7c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_74.3510000005c7c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65416
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC5530.8453589335942276No Hit
ACTGTCTCTGTCTCTTATACACATCTC4770.729179405649994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCTG303.8278458E-421.0413821
ATTTCTG250.003308217721.0413820
TTCTACA456.0813363E-721.04137820
TTGTTTT1750.021.00918218
TGGTTTT1550.021.00918218
AACGTTT250.003337776321.00918217
TCCTTTT250.003337776321.00918218
TGCATTT303.867722E-421.00918217
GCCTTTT507.257768E-821.00918218
GGCATTT507.257768E-821.00918217
GAAATTT303.867722E-421.00918217
TTCGTTT250.003337776321.00918217
TGCGTTT354.5027395E-521.00918217
TGACTTT250.003337776321.00918217
AATTTTT5100.021.00918219
ACGTTTT701.4551915E-1121.00918218
ATGATTT303.867722E-421.00918217
CAACTTT303.867722E-421.00918217
CCGCTTT303.867722E-421.00918217
TGATTTT1550.021.00918218
Produced by FastQC (version 0.11.2)