FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_71.3510000005c76a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_71.3510000005c76a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78377
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA2780.3546958929277722No Hit
ATCTGCTATCCTGTCTCTTATACACAT930.1186572591449022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCTG303.8607875E-421.02236620
ATTTCTG250.003331469821.02236620
TTTTCTG405.2562955E-621.02236421
ATTCTGT405.2562955E-621.02236420
AGCCTGT250.003343849421.00894218
TGGTTTT2000.021.00894218
TTCATTT507.305243E-821.00894217
GTCATTT250.003343849421.00894217
CACATTT250.003343849421.00894217
GATATTT250.003343849421.00894217
CCAGTTT507.305243E-821.00894217
GGACTTT250.003343849421.00894217
TCGGTTT507.305243E-821.00894217
CTCTTTT507.305243E-821.00894218
ATGGTTT507.305243E-821.00894217
CAATTTT1000.021.00894218
GAGCTTT250.003343849421.00894217
TCTATTT250.003343849421.00894217
TACATTT250.003343849421.00894217
ATAATTT250.003343849421.00894217
Produced by FastQC (version 0.11.2)