FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_7.3510000005bf65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_7.3510000005bf65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97199
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC2540.2613195609008323No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT1070.11008343707239786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCTG250.003350221821.0082418
GTCATTT354.533434E-521.0082417
GGCATTT507.3536285E-821.0082417
ATCTTTT354.533434E-521.0082418
TAAATTT354.533434E-521.0082417
AGCTTTT850.021.0082418
AATCTGT250.003350221821.0082419
TATATTT456.2387335E-721.0082417
AAATTTT1000.021.0082418
TTCGTTT558.689312E-921.0082417
TGTCTTT354.533434E-521.0082417
GTCGTTT456.2387335E-721.0082417
GGCGTTT950.021.0082417
GGACTTT456.2387335E-721.0082417
TATGTTT250.003350221821.0082417
TGATTTT1450.021.0082418
TAGCTGT456.2387335E-721.0082418
GAGCTTT250.003350221821.0082417
TCAATTT354.533434E-521.0082417
TAGTTTT701.4551915E-1121.0082418
Produced by FastQC (version 0.11.2)