FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_69.3510000005c734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_69.3510000005c734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69031
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC860.1245817096666715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC354.5226512E-521.0000023
TTGTCTG250.003348120521.00000218
TGGTTTA250.003348120521.00000210
GCGGGCT250.003348120521.00000216
TGGTCGG250.003348120521.00000210
TGGTTGT250.003348120521.00000210
TGGTTGG354.5226512E-521.00000210
TGGTTGC354.5226512E-521.00000210
TGGTTGA354.5226512E-521.00000210
TGGTCCT354.5226512E-521.00000210
TGGTCCG250.003348120521.00000210
GGTGAGG250.003348120521.00000211
CAGTTTT507.306335E-821.00000218
TGGCAGC250.003348120521.00000210
TGGCACA250.003348120521.00000210
ATCTTTT507.306335E-821.00000218
TGGCAAG250.003348120521.00000210
ACGCTGT354.5226512E-521.00000218
GAAATTT354.5226512E-521.00000217
AAATTTT507.306335E-821.00000218
Produced by FastQC (version 0.11.2)