FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_62.3510000005c65f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_62.3510000005c65f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62782
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA3450.549520563218757No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT1160.18476633429964No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC930.14813163008505623No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT760.12105380523079864No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC640.10194004651014622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT950.021.0127618
TCCTTTT303.860861E-421.0127618
GGGTCTG558.520146E-921.0127618
GCCTTTT751.8189894E-1221.0127618
GGCATTT303.860861E-421.0127617
AGCTTTT651.1823431E-1021.0127618
TGACTTT354.4930854E-521.0127617
GATATTT303.860861E-421.0127617
ATGATTT303.860861E-421.0127617
CACGTTT354.4930854E-521.0127617
TCGGTTT303.860861E-421.0127617
GTTCTGT354.4930854E-521.0127620
TGATTTT1450.021.0127618
CAATTTT701.4551915E-1121.0127618
TAGTTTT1250.021.0127618
CGGGTTT1100.021.0127617
GCAGTTT303.860861E-421.0127617
ATAGTTT354.4930854E-521.0127617
GCTGTTT303.860861E-421.0127617
TCGCTTT303.860861E-421.0127617
Produced by FastQC (version 0.11.2)