FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_61.3510000005c635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_61.3510000005c635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58237
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTCTGTCTCTTATACACA1610.2764565482425262No Hit
GACTCTATGGTGGCTGTCTCTTATACA1600.2747394268248708No Hit
GACTCTATGGTGCCTGTCTCTTATACA1160.19918608444803132No Hit
GACTCTATGGTGTCTGTCTCTTATACA930.15969229184195616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT651.1641532E-1021.0275418
GGCGTTT558.420102E-921.0275417
TTATTTT651.1641532E-1021.0275418
TGATTTT651.1641532E-1021.0275418
GTATTTT1100.021.0275418
GGATTTT1300.021.0275418
CAATTTT651.1641532E-1021.0275418
ACATTTT651.1641532E-1021.0275418
GATTTTT3050.021.0275419
ATGTTTT850.021.0275418
CTATTTT850.021.0275418
CAGTTTT1000.021.02753818
CCTATTT354.4615324E-521.02753817
GGCATTT303.8378776E-421.02753817
AGCTTTT609.931682E-1021.02753818
TCGATTT250.003316529321.02753817
AAATCTG303.8378776E-421.02753818
TTCGTTT250.003316529321.02753817
TTACTTT303.8378776E-421.02753817
TGACTTT303.8378776E-421.02753817
Produced by FastQC (version 0.11.2)