FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_6.3510000005bf4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_6.3510000005bf4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98512
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA6230.632410264739321No Hit
CTGATCGTAGCACCTGTCTCTTATACA1800.18271885658599968No Hit
CTGATCGTAGCAGCTGTCTCTTATACA1200.1218125710573331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG5100.021.0101591
CTTATCG1650.021.0101591
CTGACCG651.2187229E-1021.0101571
CTGATCG82750.021.0101571
CTGAACG558.683855E-921.0101571
CTGCTCG4050.021.0101571
TTGTTTT1350.020.99949518
TGGTTTT2700.020.99949518
TAGCATA3550.020.9994958
AGCATTG1350.020.9994959
CGATCGT2250.020.9994952
CAGCTCG250.003358660520.99949311
GGAGATT405.329206E-620.99949316
GGATGTT405.329206E-620.99949316
TCCTTTT456.2656545E-720.99949318
ATGGGGG250.003358660520.99949312
GGTTGTT354.5488625E-520.99949316
GCCTTTT1450.020.99949318
CATGAGG250.003358660520.99949311
CATGAGC250.003358660520.99949311
Produced by FastQC (version 0.11.2)