FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_59.3510000005c5f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_59.3510000005c5f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74560
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCTG507.2472176E-821.02149221
ATTTCTG405.2525083E-621.02149220
CTTTCTC250.003330768821.02149221
ACTGTTT250.003343779421.00738317
TGGTTTT1800.021.00738318
AACGTTT507.298877E-821.00738317
CCAATTT250.003343779421.00738317
AAACTGT250.003343779421.00738318
GCCTTTT950.021.00738318
AAGTACC751.8189894E-1221.0073831
GGCATTT250.003343779421.00738317
ATCTTTT456.2009894E-721.00738318
AGCTTTT1000.021.00738318
CACATTT250.003343779421.00738317
TCGATTT354.516643E-521.00738317
GAAATTT250.003343779421.00738317
AAATTTT507.298877E-821.00738318
AGGATTT354.516643E-521.00738317
GGCGTTT701.4551915E-1121.00738317
GTACCTC751.8189894E-1221.0073833
Produced by FastQC (version 0.11.2)