FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_58.3510000005c5d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_58.3510000005c5d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57841
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC2930.5065610898843381No Hit
ACGTCTCTGCCTGTCTCTTATACACAT1350.23339845438356874No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT590.10200376895281894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT1650.021.01125718
AGGTTTT1250.021.01125718
TCCTTTT354.4876426E-521.01125518
GTGTTTT1700.021.01125518
GCCTTTT651.1823431E-1021.01125518
CAGTTTT1050.021.01125518
GTCATTT250.003331218821.01125517
GGCATTT507.2133844E-821.01125517
ATCTTTT303.8576173E-421.01125518
AGCTTTT800.021.01125518
AAATTTT354.4876426E-521.01125518
GATATTT303.8576173E-421.01125517
GCGATTT405.2408705E-621.01125517
ACGTTTT701.4551915E-1121.01125518
ATGATTT250.003331218821.01125517
AGGATTT558.4928615E-921.01125517
GGCGTTT558.4928615E-921.01125517
GTACTTT250.003331218821.01125517
GGACTTT250.003331218821.01125517
GATGTTT507.2133844E-821.01125517
Produced by FastQC (version 0.11.2)