FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_55.3510000005c579.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_55.3510000005c579.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90488
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1850.20444699849703826No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT990.10940677216868536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC558.56744E-921.029921
GTTTTTC250.003328479821.029920
TTATACA558.56744E-921.029920
ATTCTGT507.2597686E-821.029920
ATTTCTG354.4930828E-521.029920
CTTCTGT456.167338E-721.029920
CTTTCTC250.003328479821.029921
TCCTTTT303.886798E-421.00663818
CAGTTTT1100.021.00663818
GGCATTT900.021.00663817
GAAATTT354.5316418E-521.00663817
TTCGTTT354.5316418E-521.00663817
CCAGTTT303.886798E-421.00663817
ACGTTTT651.2187229E-1021.00663818
TTTCTTT354.5316418E-521.00663817
ATGATTT303.886798E-421.00663817
AGGATTT303.886798E-421.00663817
GTCGTTT405.305539E-621.00663817
GGCGTTT1350.021.00663817
GTACTTT405.305539E-621.00663817
Produced by FastQC (version 0.11.2)