FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_51.3510000005c4f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_51.3510000005c4f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47580
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC5861.2316099201345103No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC2300.48339638503572935No Hit
GAGGAACAGAGCTGTCTCTTATACACA1880.39512400168137873No Hit
GAGGAACAGAGTGGGGGCTGTCTCTTA480.1008827238335435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTT701.4551915E-1121.01684418
CAGTTTT354.4569068E-521.01684418
ATCTTTT701.4551915E-1121.01684418
TTTTTTC354.4569068E-521.01684421
ACATTTT354.4569068E-521.01684418
GTCTTTT701.4551915E-1121.01684418
TAATTTT354.4569068E-521.01684418
TCATTTT701.4551915E-1121.01684418
GCATTTT950.021.01684418
GGAATTT354.4569068E-521.01684417
TTGTTTT609.858923E-1021.01684218
TGGTTTT900.021.01684218
TCCTTTT250.003317488421.01684218
GGCATTT405.1965E-621.01684217
TAAATTT303.836783E-421.01684217
AGCTTTT558.370989E-921.01684218
TGCGTTT303.836783E-421.01684217
ACGTTTT507.125345E-821.01684218
TTATTTT507.125345E-821.01684218
TGATTTT609.858923E-1021.01684218
Produced by FastQC (version 0.11.2)