FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_5.3510000005bf22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_5.3510000005bf22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46593
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC1950.4185178031034705No Hit
GAGCTACGTCTCTGTCTCTTATACACA1060.22750198527675833No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC870.18672332753847143No Hit
GAGCTACGTCTAGGGCTGTCTCTTATA680.14594466980018458No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT580.12448221835898096No Hit
GAGCTACGTCTAGCCTGTCTCTTATAC520.11160474749425879No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC250.003331952321.00000210
TGCATTT250.003331952321.00000217
GAGCGTT250.003331952321.00000216
ACGCTTT354.4820423E-521.00000217
CCTATTT250.003331952321.00000217
CTATATG250.003331952321.00000210
TAGTGTA250.003331952321.00000211
TCTAGTT1000.021.0000029
TCTAGTG1400.021.0000029
CTATAGG354.4820423E-521.00000210
TCTAGAG1000.021.0000029
TACAGGG250.003331952321.00000211
GGCGTTT558.438292E-921.00000217
TCGGTTT250.003331952321.00000217
GAAGTTT250.003331952321.00000217
TAACGTG250.003331952321.00000211
GTTCTGT250.003331952321.00000220
GATGTTT250.003331952321.00000217
CGCTTTT1000.021.00000218
GCGGTTT507.178096E-821.00000217
Produced by FastQC (version 0.11.2)