FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_47.3510000005c47a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_47.3510000005c47a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104039
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC2190.21049798633204858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTT303.9005533E-421.016
GGAGATG405.332826E-621.010
GGAGATA303.9005533E-421.010
GATGGGG456.271166E-721.011
TGGTTTT2400.021.018
GTATGTT250.003359436821.016
GGATGTA601.036824E-921.010
AACGTTT507.3969204E-821.017
GATGGAG354.5511617E-521.011
TAGCGGA701.4551915E-1121.01
GAGGTGC405.332826E-621.011
GAGGTGA250.003359436821.011
TCCTTTT303.9005533E-421.018
GGAGACA507.3969204E-821.010
GGATGGA651.2369128E-1021.010
GGAGAAG651.2369128E-1021.010
GGAGAAC250.003359436821.010
GAGAAGG250.003359436821.011
GGATGCG507.3969204E-821.010
GGATGCC507.3969204E-821.010
Produced by FastQC (version 0.11.2)