FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_46.3510000005c451.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_46.3510000005c451.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155630
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA4730.3039259782818223No Hit
TACTGCAAGGAGGCTGTCTCTTATACA1950.12529717920709377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCAA250.003362365521.0048222
ACTGCAG354.5617868E-521.0048222
ACTGCAC303.9065207E-421.0048222
ACTGCAA143800.021.0048222
TTGCAAG3000.021.0048223
CAGCAAG250.003362365521.0048223
TGCAAGG152250.021.0048224
TTCTGCA3800.021.0048221
TAATGCA1850.021.0048221
TATTGCA3000.021.0048221
TCTGCAA3800.021.0048222
ATTGCAA3000.021.0048222
ATGCAAG1850.021.0048223
ACAAGGA1350.021.0048225
CTGCAGG354.5617868E-521.0048223
CTGCACG303.9065207E-421.0048223
AATGCAA1850.021.0048222
CTGCAAG147450.021.0048223
TACTACA1350.021.0048221
ACTACAA1350.021.0048222
Produced by FastQC (version 0.11.2)