FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_45.3510000005c437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_45.3510000005c437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60607
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG303.8746535E-421.00000210
TCCTTTT558.565621E-921.00000218
TGCATTT303.8746535E-421.00000217
AGGGGGG558.565621E-921.00000212
CTATATG303.8746535E-421.00000210
ATCTTTT651.200533E-1021.00000218
GGGCATT303.8746535E-421.00000216
ACGCTGT303.8746535E-421.00000218
ACTCGAC1200.021.0000022
ACGCTAA10950.021.0000027
TCGATTT303.8746535E-421.00000217
CTATAAG303.8746535E-421.00000210
GCTAGTG1300.021.0000029
CGCCTTT303.8746535E-421.00000217
GCTAGCC1300.021.0000029
TCGGTTT303.8746535E-421.00000217
GCTGGTT303.8746535E-421.00000216
CCTCGAC558.565621E-921.0000022
TTTTCTG303.8746535E-421.00000221
GTATCTG303.8746535E-421.00000218
Produced by FastQC (version 0.11.2)