FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_44.3510000005c400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_44.3510000005c400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91341
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTC303.8585442E-421.02962320
TTTTTTC354.4941407E-521.02962121
TTTTTCT354.4941407E-521.02962121
ATTTTCT456.169448E-721.02962120
CTTTCTG250.00332897421.02962120
TTGTCTG303.887263E-421.00657718
TGCATTT303.887263E-421.00657717
ACGTCTG303.887263E-421.00657718
CACGTTT303.887263E-421.00657717
GAAGTTT405.306576E-621.00657717
TTATTTT1250.021.00657718
CCGTTTT1050.021.00657718
GCAATTT405.306576E-621.00657717
CGGGTTT800.021.00657717
GCAGTTT601.027729E-921.00657717
CCTCTGT303.887263E-421.00657719
CTTATTT303.887263E-421.00657717
TATTCTG303.887263E-421.00657719
GATTCTG303.887263E-421.00657719
CGTGTTT303.887263E-421.00657717
Produced by FastQC (version 0.11.2)