FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_41.3510000005c3be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_41.3510000005c3be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68570
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC3930.5731369403529241No Hit
TCAACCGATAGGGCTGTCTCTTATACA830.1210441884205921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT1200.021.03874421
ATTTCTG609.949872E-1021.03874420
CTCTGTC1750.021.03874420
TTTTCTG507.1629984E-821.03874221
TTTTTTC405.205071E-621.03874221
GTTTCTG507.1629984E-821.03874220
ATTTTCT354.4581313E-521.03874220
CTTTTTT8250.021.03874220
CAATTTT651.200533E-1021.0080318
ACTCTGT651.200533E-1021.0080319
TCAACCG61200.021.008031
TCCACCG1250.021.008031
TACTTTT651.200533E-1021.0080318
GTTATTT651.200533E-1021.0080317
ATCCTGT456.184091E-721.00802818
TTGTTTT950.021.00802818
TCCTTTT250.00334046521.00802818
CCAATTT250.00334046521.00802817
GCCTTTT354.5087334E-521.00802818
CAGTTTT507.274866E-821.00802818
Produced by FastQC (version 0.11.2)