FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_36.3510000005c301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_36.3510000005c301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106605
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA3960.37146475306036303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGC701.4551915E-1121.0000026
ACAGTGG17650.021.0000028
TGGTTTT2200.021.00000218
ACAGTCC4400.021.0000028
TCCTTTT354.552534E-521.00000218
CGGAGTT354.552534E-521.00000216
GGCATTT354.552534E-521.00000217
ATCTTTT651.2369128E-1021.00000218
GTGCATG303.901436E-421.00000211
CACATTT303.901436E-421.00000217
CAGTTGT1500.021.0000029
CAGTTGA1500.021.0000029
CAGTCCA558.752977E-921.0000029
CAGTCAA601.036824E-921.0000029
CAGTTAG1200.021.0000029
CAGTTAC601.036824E-921.0000029
TCGATTT303.901436E-421.00000217
AGGATTT354.552534E-521.00000217
CGGGGTT354.552534E-521.00000216
AGTAGGT601.036824E-921.00000210
Produced by FastQC (version 0.11.2)