FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_35.3510000005c2e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_35.3510000005c2e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127201
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA8600.6760953137160871No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA1330.10455892642353441No Hit
CACAGAAGGACTGGGGCTGTCTCTTAT1330.10455892642353441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC950.021.0137720
TTTTTTC405.3179865E-621.0137721
GTTTTCT456.254304E-721.0137720
ATTTCTG751.8189894E-1221.0137720
ATTTTCT303.8898923E-421.0137720
ATACACA800.021.0137721
CACACAA456.278042E-721.0055071
CACAAGG456.278042E-721.0055073
CACAAAA507.4091076E-821.0055071
ACACAAG456.278042E-721.0055072
CAAAAGG507.4091076E-821.0055073
ACAGAAG114000.021.0055052
CACCGAA4600.021.0055051
TCAGAAG4350.021.0055052
ATAGAAG2150.021.0055052
AAGAAGG601.0404619E-921.0055053
CAAAGAA601.0404619E-921.0055051
CATAGAA2150.021.0055051
CAGAAGG118300.021.0055053
CACAGAA114200.021.0055051
Produced by FastQC (version 0.11.2)