FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_33.3510000005c2a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_33.3510000005c2a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53502
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA1580.2953160629509177No Hit
AACGGAATGGCTGCTGTCTCTTATACA1140.21307614668610522No Hit
AACGGAATGGCTCTGTCTCTTATACAC1080.2018616126499944No Hit
AACGGAATGGCTCCTGTCTCTTATACA1030.19251616761990206No Hit
AACGGAATGGCTGTCTCTTATACACAT800.14952712048147732No Hit
AACGGAATGGCTACTGTCTCTTATACA570.10653807334305261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT250.003327174321.01216117
GTGTTTT1600.021.01216118
GGGTTTT1500.021.01216118
GTCATTT250.003327174321.01216117
GGCATTT250.003327174321.01216117
ATCTTTT250.003327174321.01216118
AGCTTTT405.226826E-621.01216118
GAAATTT250.003327174321.01216117
AAATTTT609.968062E-1021.01216118
TTCGTTT250.003327174321.01216117
AGGATTT405.226826E-621.01216117
GTCGTTT405.226826E-621.01216117
CCTGTTT303.8512956E-421.01216117
GGACTTT405.226826E-621.01216117
TTTCTGT1200.021.01216121
TCGGTTT250.003327174321.01216117
GAAGTTT250.003327174321.01216117
ACGCAAT405.226826E-621.0121612
TTTTCTG303.8512956E-421.01216121
TTTTTTC303.8512956E-421.01216121
Produced by FastQC (version 0.11.2)