FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_31.3510000005c26f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_31.3510000005c26f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86665
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA1460.16846477816881095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTCT303.865878E-421.02195421
CTTTCTC250.00333467521.02195421
TTGTTTT1950.020.99769418
ATCTTTT1100.020.99769418
GCAAGTG1950.020.9976949
CGCTTTT1100.020.99769418
GCAATTG1850.020.9976949
GCAATGT1950.020.9976949
GCAACAC558.707502E-920.9976949
CAAGGTA558.707502E-920.99769410
CGCAATG7400.020.9976948
CGCAAAA3900.020.9976948
CAAGTGT558.707502E-920.99769410
CAAGCAT558.707502E-920.99769410
ACTGTTT405.320924E-620.99769217
TTGTCTG250.003357105420.99769218
CAAGATA303.8961877E-420.99769210
TCCTTTT354.543738E-520.99769218
CAATGTG900.020.99769210
CAATGTA405.320924E-620.99769210
Produced by FastQC (version 0.11.2)