FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_30.3510000005c246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_30.3510000005c246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80353
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC3310.41193234851218996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT558.53106E-921.02992618
AAACTTT250.003325247421.02992617
TGCATTT405.242646E-621.02992617
TGGTTCT250.003325247421.02992618
GTGTTTT2000.021.02992618
TCGATTT250.003325247421.02992617
GAAATTT250.003325247421.02992617
AAATTTT507.234121E-821.02992618
TTCGTTT250.003325247421.02992617
GCGATTT405.242646E-621.02992617
TGTCTTT250.003325247421.02992617
AGGATTT507.234121E-821.02992617
CCTGTTT250.003325247421.02992617
GCGGTTT900.021.02992617
TAGCTTT405.242646E-621.02992617
TTTTCTG800.021.02992621
TGTTCTG250.003325247421.02992619
ATGGTTT507.234121E-821.02992617
AGGGTTT850.021.02992617
TTCTGTC2000.021.02992621
Produced by FastQC (version 0.11.2)