FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_27.3510000005c1e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_27.3510000005c1e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66466
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC507.240669E-821.015061
TTTGACC1650.021.015061
TTCGCCC2600.021.015061
TTCGTCC456.158334E-721.015061
TTCGACC54750.021.015061
TGCGACC3400.021.015061
TCCGACC1300.021.015061
TTCGGCC456.158334E-721.015061
CGACAGG303.8810458E-420.99924910
TCCTTTT507.298877E-820.99924918
TTCATTT250.00334752820.99924917
GAGGTAC250.00334752820.99924911
CCAATTT250.00334752820.99924917
GAGAAGG303.8810458E-420.99924911
CGATAGT303.8810458E-420.99924910
CGATAGG250.00334752820.99924910
CGATACG507.298877E-820.99924910
TGACTTT250.00334752820.99924917
CGCCTTT303.8810458E-420.99924917
AACGATC2450.020.9992498
Produced by FastQC (version 0.11.2)