FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_26.3510000005c1cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_26.3510000005c1cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91997
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC8350.9076382925530181No Hit
ACTGTCTCTGTCTCTTATACACATCTC7430.8076350315771166No Hit
ACTGTCTGTCTCTTATACACATCTCCG970.1054382208115482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCTG558.574716E-921.02941320
TTTTCTG303.8590937E-421.02941121
GTTTCTG354.4949364E-521.02941120
TTCTACA303.8590937E-421.02941120
CAGTTTT1300.021.0065318
AAATTTT1150.021.0065318
GATGTTT651.2187229E-1021.0065317
GTGATTT651.2187229E-1021.0065317
AGGTTTT2300.021.0065318
AGCGTTT651.2187229E-1021.0065317
CCCTTTT651.2187229E-1021.0065318
AACGTTT405.30736E-621.00652917
AAACTTT250.003350453221.00652917
TTCATTT405.30736E-621.00652917
TGCATTT303.8876123E-421.00652917
CCTATTT250.003350453221.00652917
AGCTTTT1450.021.00652918
TATATTT303.8876123E-421.00652917
TTCGTTT303.8876123E-421.00652917
TGACTTT405.30736E-621.00652917
Produced by FastQC (version 0.11.2)