FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_24.3510000005c18a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_24.3510000005c18a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60412
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC5250.8690326425213535No Hit
GTGTAGTGTGGCTGTCTCTTATACACA3660.6058398993577435No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC1800.297954048864464No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC1670.27643514533536384No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC1630.26981394424948685No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC1500.2482950407203867No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC1450.24001853936304046No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC1330.2201549361054095No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA1230.20360193339071705No Hit
GTGTAGTGTGGACCTGTCTCTTATACA940.15559822551810898No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT820.13573462226047806No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC740.1224922200887241No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC610.10097331655962391No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCTG303.8579234E-421.01325618
TGGTTTT1300.021.01325618
AACGTTT303.8579234E-421.01325617
GCCTTTT250.003331100621.01325618
CAGTTTT701.4551915E-1121.01325618
CAGTTTC303.8579234E-421.01325618
GGCATTT250.003331100621.01325617
AGCTTTT558.5019565E-921.01325618
TATATTT405.2430296E-621.01325617
AAATTTT405.2430296E-621.01325618
TGCGTTT354.48867E-521.01325617
AATTTTT2300.021.01325619
ACGTTTT900.021.01325618
AGGATTT354.48867E-521.01325617
GTCGTTT303.8579234E-421.01325617
GGCGTTT601.0022632E-921.01325617
TATGTTT405.2430296E-621.01325617
TGATTTT1000.021.01325618
GATGTTT250.003331100621.01325617
CGCTTTT558.5019565E-921.01325618
Produced by FastQC (version 0.11.2)