FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_22.3510000005c154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_22.3510000005c154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89232
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCGCTGTCTCTTATACA2310.25887573964497046No Hit
GTCAGAACTACCCCTGTCTCTTATACA2160.24206562668101128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCTG651.2187229E-1021.01065621
GTTTTCT303.881199E-421.01065620
TTATACA751.8189894E-1221.01065621
CTTCTGT250.003345862521.01065620
ACCGGTG1250.020.9988810
GGATGTT303.8959438E-420.9988816
GCGGGAT303.8959438E-420.9988815
AAACTAC303.8959438E-420.998885
CCGGAGG601.031367E-920.9988811
CGATATT303.8959438E-420.9988816
AGGATTT303.8959438E-420.9988817
CCTGTTT303.8959438E-420.9988817
CTGGGGG303.8959438E-420.9988812
ACCATAA303.8959438E-420.9988810
TCGGTGT303.8959438E-420.9988815
GTCAAAA303.8959438E-420.998881
ACCGCTC303.8959438E-420.9988810
ACCGTTG601.031367E-920.9988810
GGCGATT303.8959438E-420.9988816
ACCGCCT303.8959438E-420.9988810
Produced by FastQC (version 0.11.2)