FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_21.3510000005c13a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_21.3510000005c13a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105155
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC1580.15025438638200753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTT303.9009482E-421.00000217
TGCATTT303.9009482E-421.00000217
TGGTCGG303.9009482E-421.00000210
TGGTCGC303.9009482E-421.00000210
TGGTTGA303.9009482E-421.00000210
TGGCATG558.749339E-921.00000210
TGGTCCG558.749339E-921.00000210
TGGTTCA303.9009482E-421.00000210
GCCTTTT601.036824E-921.00000218
TGGCAGA303.9009482E-421.00000210
ATCTCTG303.9009482E-421.00000218
TGGCAAC303.9009482E-421.00000210
CCAGTTT303.9009482E-421.00000217
TGGTAGG601.036824E-921.00000210
CTCTTTT558.749339E-921.00000218
CCTCGAA303.9009482E-421.0000022
ATGGCTG303.9009482E-421.00000217
TTTTTTC601.036824E-921.00000221
CGGATTT558.749339E-921.00000217
GCAATTT303.9009482E-421.00000217
Produced by FastQC (version 0.11.2)