FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_19.3510000005c0e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_19.3510000005c0e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93181
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT507.34417E-821.00859518
ACGCTTT250.003348869721.00859517
GCCTTTT800.021.00859518
CCTATTT507.34417E-821.00859517
TCGATTT405.3040767E-621.00859517
TGCGTTT405.3040767E-621.00859517
TTACTTT303.885562E-421.00859517
TGACTTT250.003348869721.00859517
CCAGTTT405.3040767E-621.00859517
GCGATTT651.2187229E-1021.00859517
ACGTTTT1000.021.00859518
CCTGTTT303.885562E-421.00859517
GTACTTT303.885562E-421.00859517
GGACTTT303.885562E-421.00859517
GGTCTTT507.34417E-821.00859517
TGTTCTG250.003348869721.00859519
ACCGTTT250.003348869721.00859517
ACACTTT250.003348869721.00859517
GAGCTTT303.885562E-421.00859517
CGGATTT507.34417E-821.00859517
Produced by FastQC (version 0.11.2)