FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_18.3510000005c0ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_18.3510000005c0ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72578
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA2380.32792306208492933No Hit
GCATTGGATACCGGCTGTCTCTTATAC2360.32516740610102235No Hit
GCATTGGATACCCTGTCTCTTATACAC1780.24525338256772025No Hit
GCATTGGATACCAGCTGTCTCTTATAC1160.1598280470666042No Hit
GCATTGGATACCGCCTGTCTCTTATAC1100.1515610791148833No Hit
GCATTGGATACCCGCTGTCTCTTATAC1080.14880542313097633No Hit
GCATTGGATACCTGCTGTCTCTTATAC1080.14880542313097633No Hit
GCATTGGATACCGTCTGTCTCTTATAC1040.1432941111631624No Hit
GCATTGGATACCACCTGTCTCTTATAC940.12951583124362753No Hit
GCATTGGATACCCCCTGTCTCTTATAC870.11987103529995315No Hit
GCATTGGATACCATCTGTCTCTTATAC810.11160406734823225No Hit
GCATTGGATACCCCTGTCTCTTATACA730.10058144341260437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTGT456.185892E-721.01103618
TGCATTT405.272035E-621.01103617
CAGTTTT1100.021.01103618
ATCTTTT405.272035E-621.01103618
AATCTGT250.00333955621.01103619
TCGATTT405.272035E-621.01103617
CGCCTTT250.00333955621.01103617
CCAGTTT250.00333955621.01103617
TGTCTTT250.00333955621.01103617
TTACTGT250.00333955621.01103618
AGGATTT354.5084438E-521.01103617
GGCGCTG250.00333955621.01103617
GTTCTTT250.00333955621.01103617
CTCTTTT405.272035E-621.01103618
TTTTCTG405.272035E-621.01103621
GTTTCTG456.185892E-721.01103620
TCAATTT250.00333955621.01103617
GTTTTCT250.00333955621.01103620
TAGTTTT1100.021.01103618
GAGTCTG250.00333955621.01103618
Produced by FastQC (version 0.11.2)