FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_17.3510000005c0a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_17.3510000005c0a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45410
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC3330.7333186522792337No Hit
AACGTGCGTATGGCTGTCTCTTATACA1720.3787711957718564No Hit
AACGTGCGTATGGGCTGTCTCTTATAC1590.3501431402774719No Hit
AACGTGCGTATGCTGTCTCTTCTACAC1080.23783307641488657No Hit
AACGTGCGTATCTGTCTCTTATACACA1020.22462012772517065No Hit
AACGTGCGTATGCCTGTCTCTTATACA830.18277912354107026No Hit
AACGTGCGTATGAGCTGTCTCTTATAC710.1563532261616384No Hit
AACGTGCGTATGGCCTGTCTCTTATAC650.14314027747192248No Hit
AACGTGCGTATGACCTGTCTCTTATAC650.14314027747192248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTT405.224256E-621.00000216
GTATGGC3400.021.0000028
GTATGCT2100.021.0000028
GTATGCG3400.021.0000028
TATGTTG751.8189894E-1221.0000029
TATGCGA800.021.0000029
TATGTCG800.021.0000029
ATGGCGC405.224256E-621.00000210
ATGGCGA405.224256E-621.00000210
TAGTTTT751.8189894E-1221.00000218
TATGAGC850.021.0000029
ATGGAGG800.021.00000210
ATGGACG405.224256E-621.00000210
TGGGGGG751.8189894E-1221.00000211
GAATTTT850.021.00000218
GCGTTTT850.021.00000218
ATGTTTT1050.021.00000218
TTGGTTT405.224256E-621.00000217
ATTCTGT405.224256E-621.00000220
GCATTTT1050.021.00000218
Produced by FastQC (version 0.11.2)