FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_15.3510000005c061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_15.3510000005c061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67846
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA4550.6706364413524747No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT2020.29773310143560416No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT990.14591869822834067No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC930.1370751407599564No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT870.1282315832915721No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA860.12675765704684136No Hit
TGGCATAGCTGAGGGCTGTCTCTTATA750.11054446835480354No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT700.10317483713114997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGCG303.8812312E-421.00000210
GATGGCG303.8812312E-421.00000211
GCCTTTT651.200533E-1021.00000218
ATCTTTT303.8812312E-421.00000218
CTGAGCA751.8189894E-1221.0000029
CTGAGAA751.8189894E-1221.0000029
GGCATAG58900.021.0000022
TGCGTTT303.8812312E-421.00000217
TGACTTG303.8812312E-421.00000210
GATATTT303.8812312E-421.00000217
CGCCTTT303.8812312E-421.00000217
GTCGTTT303.8812312E-421.00000217
TGACCAA303.8812312E-421.00000210
GGCGCGT303.8812312E-421.00000215
GGCGTAG303.8812312E-421.0000022
GGCCGGT303.8812312E-421.00000215
AAGAGTT303.8812312E-421.00000216
GCGGTTT751.8189894E-1221.00000217
TTTTCTG651.200533E-1021.00000221
GAGCCGC303.8812312E-421.00000211
Produced by FastQC (version 0.11.2)