FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_14.3510000005c055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_14.3510000005c055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68570
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA4080.5950123960915853No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT1370.1997958290797725No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC1240.18083710077293277No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT890.12979437071605657No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC720.10500218754557386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCTG601.0095391E-921.0131420
ATGCCCT354.500291E-521.013141
ATGCTCT60650.021.013141
ATTTCTG405.260441E-621.0131420
AAGCTCT701.4551915E-1121.013141
CTTTTTT12550.021.0131420
ACGCTCT651.200533E-1021.0131381
ATGATCT850.021.0131381
ATGCACT651.200533E-1021.0131381
ATTCTCT1300.021.0131381
ATACACA558.554707E-921.01313821
TTGCGGG5900.020.9978149
ACTGTTG651.200533E-1020.9978125
CGCTGGG250.003349924520.99781212
TGGTTTT1750.020.99781218
TCCTTTT250.003349924520.99781218
GCGGGCC354.5257304E-520.99781211
TGCACTG651.200533E-1020.9978122
TGTTGCT13850.020.9978127
TGTTGCA10600.020.9978127
Produced by FastQC (version 0.11.2)