FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_11.3510000005bfec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_11.3510000005bfec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96338
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA1080.11210529593722103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTT1050.021.00831418
GCGTTTT2100.021.00831418
CGATTTT1050.021.00831418
ACTGTTT405.3077765E-621.00831217
TCCTTTT405.3077765E-621.00831218
GCCTTTT1000.021.00831218
GGCATTT250.00334994421.00831217
AGCTTTT1000.021.00831218
TGCGCTG250.00334994421.00831217
TGCGTTT507.3516276E-821.00831217
CGCCTTT250.00334994421.00831217
ATGATTT250.00334994421.00831217
TCGGCTG250.00334994421.00831217
GATGTTT405.3077765E-621.00831217
AGGGTTT405.3077765E-621.00831217
CAATTTT900.021.00831218
GAGCTTT405.3077765E-621.00831217
TGTTTCT250.00334994421.00831219
GGTTCTG250.00334994421.00831219
CATTTTT3250.021.00831219
Produced by FastQC (version 0.11.2)