FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate2_10.3510000005bfc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate2_10.3510000005bfc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72449
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC5710.7881406230589795No Hit
ACGTCTCTGCCTGTCTCTTATACACAT2140.29538019848445113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTGT601.0077201E-921.02351220
TTTTCTG303.8554394E-421.02351221
TTTTTTA250.003327993221.02351221
TTTTTCT354.4876422E-521.02351221
TCTACAC250.003327993221.02351221
TTCTACA250.003327993221.02351220
CTTCTGT250.003327993221.02351220
CTTTCTG250.003327993221.02351220
TCCTTTT405.2762425E-621.00898618
GGGTTTT3800.021.00898618
ATCTTTT651.200533E-1021.00898618
TGCGTTT405.2762425E-621.00898617
ACGTCTC63500.021.0089861
ACGTTTC651.200533E-1021.0089861
AGGATTT405.2762425E-621.00898617
CGCTTTT1100.021.00898618
CCGTTTT558.592906E-921.00898618
TAGTTTT950.021.00898618
GCAGTTT558.592906E-921.00898617
GACGTTT405.2762425E-621.00898617
Produced by FastQC (version 0.11.2)