FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_95.3510000005be66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_95.3510000005be66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28230
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC700.2479631597591215No Hit
TAGCAGACTGGACCTGTCTCTTATACA470.16648955012398156No Hit
TAGCAGACTGGAGCTGTCTCTTATACA440.15586255756287637No Hit
TAGCAGACTGGAAGCTGTCTCTTATAC400.14169323414806942No Hit
TAGCAGACTGAAAAAAAAAAAAAAAAA370.13106624158696423No Hit
TAGCAGACTGGCTGTCTCTTATACACA340.12043924902585901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT405.048729E-621.0284218
TGGTTTT250.003273863821.0284218
GTGTTTT1000.021.0284218
CAGTTTT405.048729E-621.0284218
AAATTTT303.7692033E-421.0284218
GGCGTTT303.7692033E-421.0284217
TAAGTTT250.003273863821.0284217
TTATTTT405.048729E-621.0284218
TGATTTT354.355683E-521.0284218
CTCTTTT303.7692033E-421.0284218
CGCTTTT354.355683E-521.0284218
GCGGTTT405.048729E-621.0284217
TTTTCTG405.048729E-621.0284221
TTTTTTC303.7692033E-421.0284221
TGTTTTT2100.021.0284219
GTTTCTG354.355683E-521.0284220
CATTTTT1500.021.0284219
CTGATTT250.003273863821.0284217
TAGTTTT609.276846E-1021.0284218
ACATTTT303.7692033E-421.0284218
Produced by FastQC (version 0.11.2)