FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_93.3510000005be23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_93.3510000005be23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19807
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGACGCTGTCTCTTATACACATC240.1211692835866108No Hit
ATCTCGACGCTCTGTCTCTTATACACA210.10602312313828445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT557.719791E-921.00000218
ACGCTAT4400.021.0000027
TCGACGC18250.021.0000024
GCTAGTC354.316488E-521.0000029
GCTAGCC354.316488E-521.0000029
TGTTTTT1400.021.00000219
TTCGACG557.719791E-921.0000023
TAGTTTT354.316488E-521.00000218
GCTACTC354.316488E-521.0000029
CGCTAGC1400.021.0000028
CGCTACA950.021.0000028
CGCTAAC701.2732926E-1121.0000028
CTAGGGG354.316488E-521.00000210
GCTACGA354.316488E-521.0000029
GCTACCG557.719791E-921.0000029
CCATTTT354.316488E-521.00000218
GCTAAGT354.316488E-521.0000029
CCTTTTT557.719791E-921.00000219
CTACGGG354.316488E-521.00000210
CGACGCT18250.021.0000025
Produced by FastQC (version 0.11.2)