FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_88.3510000005bd9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_88.3510000005bd9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15530
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC1290.8306503541532518No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC580.37347070186735354No Hit
GTTGAGCTTGACGCTGTCTCTTATACA370.23824855119124275No Hit
GTTGAGCTTGACCCTGTCTCTTATACA310.19961365099806827No Hit
GTTGAGCTTGACGGCTGTCTCTTATAC190.12234385061171925No Hit
GTTGAGCTTGACGGGGGGGGTTTTTTT170.10946555054732775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTTT557.392373E-921.00000218
TTGACTA850.021.0000028
TTGACCT557.392373E-921.0000028
TTGACCG1100.021.0000028
TTGACCC557.392373E-921.0000028
TTGACCA557.392373E-921.0000028
TTGACAA557.392373E-921.0000028
CTTGACA2550.021.0000027
TGACGCG557.392373E-921.0000029
TGACGAG850.021.0000029
TTGTTTT608.4946805E-1021.018
TGGTTTT608.4946805E-1021.018
GTTGAGC12250.021.01
GGTGAGC751.8189894E-1221.01
CTGAGCT404.8660913E-621.02
TGCGCTT800.021.03
TGCGTTT303.697677E-421.017
AATTTTT751.8189894E-1221.019
ACGTTTT303.697677E-421.018
TGACCGG455.592465E-721.09
Produced by FastQC (version 0.11.2)