FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_87.3510000005bd67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_87.3510000005bd67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26848
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA470.1750595947556615No Hit
ACTTCGTCAGCTGTCTCTTATACACAT440.16388557806912993No Hit
ACTTCGTCAGCTCTGTCTCTTATACAC350.13036352800953516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCC1100.021.0000028
TTGTTTT558.039933E-921.00000218
GTGTTTT850.021.00000218
CAGTTTT354.3911277E-521.00000218
ATCTTTT354.3911277E-521.00000218
AGCTTTA354.3911277E-521.0000029
AGCTTGT850.021.0000029
AGCTTCT354.3911277E-521.0000029
AGCTCAC354.3911277E-521.0000029
AGCTTAG701.2732926E-1121.0000029
GCTAGGC354.3911277E-521.00000210
GGCGTTT354.3911277E-521.00000217
AGCTAGC354.3911277E-521.0000029
AGCTACC354.3911277E-521.0000029
TAGTTTT354.3911277E-521.00000218
ACTTTTT558.039933E-921.00000219
TCAGCTC5050.021.0000027
TGCTTTT558.039933E-921.00000218
CCATTTT354.3911277E-521.00000218
GCTTGTG354.3911277E-521.00000210
Produced by FastQC (version 0.11.2)