FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_86.3510000005bd4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_86.3510000005bd4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24021
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA1530.6369426751592357No Hit
TGTTGCCGAACAGCTGTCTCTTATACA480.19982515299113274No Hit
TGTTGCCGAACACCTGTCTCTTATACA420.17484700886724117No Hit
TGTTGCCGAACTGTCTCTTATACACAT260.10823862453686359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC303.7811184E-421.010
ACAGTCG303.7811184E-421.010
ACAGCAG303.7811184E-421.010
TTTTGCC405.0561284E-621.01
ACAAAGG303.7811184E-421.010
AACAATG609.240466E-1021.09
ACACGGC405.0561284E-621.010
GCCTTTT354.3663866E-521.018
AACAAGG609.240466E-1021.09
AACAACG455.867096E-721.09
AACAACA303.7811184E-421.09
AACAAAT354.3663866E-521.09
TTACCGA405.0561284E-621.03
TTATTTT455.867096E-721.018
TGATTTT455.867096E-721.018
TGTTTTT2100.021.019
TTCTGTC354.3663866E-521.021
GTATTTT354.3663866E-521.018
TGCTGCC303.7811184E-421.01
ACATTTT303.7811184E-421.018
Produced by FastQC (version 0.11.2)